Journal: Science Advances
Article Title: Innate immune sensing of dietary alcohol ignites inflammation to drive alcohol-related disease
doi: 10.1126/sciadv.aea3979
Figure Lengend Snippet: ( A and B ) Real-time analysis of cell death in WT (A) pBMDMs and (B) iBMDMs treated with the indicated doses of ethanol (EtOH). The representative images of cell death (red color) at 9 hours are shown (below). Scale bars, 100 μm. ( C ) Heatmap depicting differentially regulated pathways in WT iBMDMs compared with pBMDMs. NES, normalized enrichment scores. ( D ) Real-time analysis of cell death in WT pBMDMs following stimulation with 0.6 M EtOH in the presence of PBS, IFN-γ (50 ng/ml), or IFN-β (20 ng/ml) added 12 hours before EtOH. The representative images of cell death (red color) at 12 hours are shown (right). Scale bar, 100 μm. ( E ) Immunoblot analysis of LDHA and HMGB1 in WT pBMDMs stimulated with EtOH plus IFN-β. Densitometric quantification of the representative blot is shown, relative to the untreated (UT) group. ( F and G ) Real-time analysis of cell death in WT pBMDMs infected with (F) IAV or (G) HSV-1 in the presence or absence of EtOH. ( H ) Real-time analysis of cell death in WT and Ifnar1 −/− pBMDMs treated with EtOH plus IFN-β. Data are representative of at least two (C) or three [(A), (B), (D), (E), (F), (G), and (H)] independent experiments. * P < 0.05, ** P < 0.01, and **** P < 0.0001. Analysis was performed using one-way analysis of variance (ANOVA) [(D), (F), and (G)] and t test (H). Data are shown as mean ± SEM [(A), (B), (D), (F), (G), and (H)].
Article Snippet: Human HSV-1 (HF strain) (American Type Culture Collection, VR-260) was purchased and propagated in Vero cells (Korean Cell Line Bank, 10081).
Techniques: Western Blot, Infection
ATCC is a verified supplier 
ATCC manufactures this product 
![( A and B ) Real-time analysis of cell death in WT (A) pBMDMs and (B) iBMDMs treated with the indicated doses of ethanol (EtOH). The representative images of cell death (red color) at 9 hours are shown (below). Scale bars, 100 μm. ( C ) Heatmap depicting differentially regulated pathways in WT iBMDMs compared with pBMDMs. NES, normalized enrichment scores. ( D ) Real-time analysis of cell death in WT pBMDMs following stimulation with 0.6 M EtOH in the presence of PBS, IFN-γ (50 ng/ml), or IFN-β (20 ng/ml) added 12 hours before EtOH. The representative images of cell death (red color) at 12 hours are shown (right). Scale bar, 100 μm. ( E ) Immunoblot analysis of LDHA and HMGB1 in WT pBMDMs stimulated with EtOH plus IFN-β. Densitometric quantification of the representative blot is shown, relative to the untreated (UT) group. ( F and G ) Real-time analysis of cell death in WT pBMDMs infected with (F) IAV or <t>(G)</t> <t>HSV-1</t> in the presence or absence of EtOH. ( H ) Real-time analysis of cell death in WT and Ifnar1 −/− pBMDMs treated with EtOH plus IFN-β. Data are representative of at least two (C) or three [(A), (B), (D), (E), (F), (G), and (H)] independent experiments. * P < 0.05, ** P < 0.01, and **** P < 0.0001. Analysis was performed using one-way analysis of variance (ANOVA) [(D), (F), and (G)] and t test (H). Data are shown as mean ± SEM [(A), (B), (D), (F), (G), and (H)].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8059/pmc13068059/pmc13068059__sciadv.aea3979-f1.jpg)